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KMID : 1098420190270060419
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Korean Journal of Medicinal Crop Science
2019 Volume.27 No. 6 p.419 ~ p.426
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Purification and Identification of Antioxidant Compounds from Dolichos lablab L. Seeds
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Kwon Nam-Woo
Kim Jae-Yeon
Cho Yong-Beom
Hwang Bang-Yeon
Kim Jun-Gu
Woo Sun-Hee
Lee Moon-Soon
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Abstract
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Background: This study aimed to identify antioxidant compounds from the seeds of Dolichos lablab L. by bioassay-guided isolation and recrystallization.
Methods and Results: The water layer of D. lablab L. seed extract inhibits intracellular reactive oxygen species (ROS) expressing the 2’,7’-dichlorofluorescein diacetate (DCF-DA), Cu/Zn superoxide dismutase (SOD) and catalase genes, as determined by quantitative real-time PCR (qRTPCR).
Two compounds were purified from the water layer of the seeds of D. lablab L. using column chromatography and prep-high performance liquid chromatography (HPLC). Using nuclear magnetic resonance (NMR) and electrospray Ionization mass spectrometry (ESI-MS), their chemical structures were identified as 5-[(2-acetyl-2,3-dihydro-1H-indazol-1-yl)carbonyl]-4,5-dihydro- 3H?furan-2-one (C14H14N2O4) and stachyose.
Conclusions: Two active antioxidant compounds were purified from the seed extract of D. lablab L. seed extract and the structures of these compounds were identified as C14H14O4N2 and stachyose.
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KEYWORD
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Dolichos lablab L., Chemical Structures, Reactive Oxygen Species Inhibitory Effect, Seed Extract, C14H14O4N2, Stachyose
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